Tuesday, 29 September 2015



GENERAL HISTOLOGY NOTES

MEANING AND SCOPE OF HISTOLOGY
Animal and plant bodies are made up of several cells, known generally as the basic unit of life, which aggregate and coordinate together as tissues. The study of these tissues is referred to as histology. The word Histology is derived from two Greek words (i) “Histos” and (ii) “logia”. The Greek word “Histos” means “Tissues” and “Logia” means “Study of”. These two put together means “Study of Tissues”. Thus Histology is described as the study of tissues. Hickman et al (2001) describes a tissue as “an aggregation of cells and cell products of similar structure and embryonic origin performing common functions”. In a similar manner that cells make tissues, so also organs and organ systems are made up of tissues. The scope of histology therefore consists of the following:
i.                     Cytology – the study of cells.
ii.                   Histology – the study of tissues.
iii.                  Organology – the study of organ and organ systems.
iv.                 Gross Anatomy – the study of the whole or component part of animals.
The study of cells (Cytology) has been dealt with extensively in another course (BIO 201 – Cell Biology). The student is advised to review that course for a good grounding for the basis of histology. These lectures may therefore focus more on tissues and their higher organizations including organs and organ systems especially of the mammalian body. This makes it extremely important for the student of histology to also review courses that have dealt with mammalian body especially ZOO 102 (Organization of Mammalian Body) and ZOO 303 (Comparative Vertebrate Anatomy).
TISSUES
The body of animals consists of different types of tissues. These mainly include the following:
(i)                  Epithelial Tissues.
(ii)                Connective Tissues.
(iii)               Muscle Tissues
(iv)              Nervous Tissues
Epithelial Tissues – Structure, Types and Functions
Epithelial tissue cover the surfaces various structures in the body. They form both internal and external coverings. An epithelial tissue that cover surfaces on the interior of the structure is known as endothelium while the one covering external surface of a structure is called epithelium. Each epithelial Tissue is made up of cells that are arranged in closely packed fashion. They may be arranged as one or more layers. Epithelial cells are packed tightly together, with almost no intercellular spaces and only a small amount of intercellular substance. An epithelial tissue, irrespective of its type, is usually separated from the underlying tissue by a thin sheet of connective tissue called basement membrane. The basement membrane provides structural support for the epithelium and also binds it to adjoining structures.
The types of epithelial tissues are identified on the following bases:
i.                     The shape and function of their cells (Squamous, cuboidal, columnar or transitional)
ii.                   The number of layers they have (Simple if only one layer and Stratified if more than one layer).
Simple epithelium
As stated above, simple epithelia can be subdivided according to the shape and function of their cells. The following can therefore be recognized:
Squamous cells have the appearance of thin, flat plates. The shape of the nucleus usually corresponds to the cell form and help to identify the type of epithelium. Squamous cells, for example, tend to have horizontal flattened, elliptical nuclei because of the thin flattened form of the cell. They form the lining of cavities such as the mouth, blood vessels, heart and lungs and make up the outer layers of the skin.
As their name implies, cuboidal cells are roughly cuboidal in shape. Each cell has a spherical nucleus in the centre. Cuboidal epithelium is found in glands and in the lining of the kidney tubules as well as in the ducts of the glands. They also constitute the germinal epithelium which produces the egg cells in the female ovary and the sperm cells in the male testes.
The cells that form columnar epithelia are elongated and column-shaped since they are taller than they are wide. The nuclei are elongated and are usually located near the base of the cells. Columnar epithelium forms the lining of the stomach and intestines. Some columnar cells are specialised for sensory reception such as in the nose, ears and the taste buds of the tongue. Goblet cells (unicellular glands) are found between the columnar epithelial cells of the duodenum. They secrete mucus or slime, a lubricating substance which keeps the surface smooth.
Some Columnar Epithelia may be ciliated, the presence of cilia being a surface specialization.  Ciliated columnar epithelia cells are usually simple. These cilia are capable of rapid, rhythmic, wavelike beatings in a certain direction. This movement of the cilia in a certain direction causes the mucus, which is secreted by the goblet cells, to move (flow or stream) in that direction. Ciliated epithelium is usually found in the air passages like the nose. It is also found in the uterus and Fallopian tubes of females. The movement of the cilia propels the ovum to the uterus.
Some columnar epithelia may be glandular in function. Columnar epithelium with goblet cells is called glandular epithelium. Some parts of the glandular epithelium consist of such a large number of goblet cells that there are only a few normal epithelial cells left. Columnar and cuboidal epithelial cells often become specialised as gland cells which are capable of synthesising and secreting certain substances such as enzymes, hormones, milk, mucus, sweat, wax and saliva. Most glands are multicellular including salivary glands.
In places where body linings have to withstand wear and tear, the epithelia are composed of several layers of cells and are therefore called stratified epithelium. The top cells are flat and scaly and it may or may not be keratinized. Keratinzed cells have deposit of a tough, resistant protein called keratin. The mammalian skin is an example of dry, keratinised, stratified epithelium. Conversely, the lining of the mouth cavity is an example of an unkeratinisied, stratified epithelium.
1.       Protection – Some epithelial cells such as those from the skin protect underlying tissue from mechanical injury, harmful chemicals and invasion by microorganisms such as bacteria. They also protect the skin from excessive loss of water.
2.       Sensation – Some epithelia function in the perception of stimuli by their possessors. Specialised epithelial tissues containing sensory nerve endings are found in the skin, eyes, ears, nose and on the tongue.
  1. Secretion – As already mentioned, some epithelia are specialized to secret specific chemical substances such as enzymes, hormones and lubricating fluids. These epithelia are found in glands.
  2. Absorption – Some epithelial cells that line the small intestine, for example, absorb nutrients from the digestion of food.
  3. Excretion – Epithelia tissues found the kidney and the skin are examples of those that functions in excretion. The epithelial tissues found in the kidney, for instance excrete waste products from the body and reabsorb needed materials from the urine. Also, sweat is also excreted from the body by epithelial cells in the sweat glands.
  4. Diffusion - Simple epithelium are functionally ideal for the diffusion of gases for example those found in the walls of capillaries and lungs. Some epithelia are therefore important in the exchange of respiratory gases. This is because of their characteristic thing nature.
  5. Cleaning – Some ciliated epithelia assist in removing dust particles and foreign bodies that found their ways into air passages.
  6. Reduction of Friction - Smooth, tightly-interlocking, epithelial cells lining the entire circulatory system of mammals and reduce friction between the blood and the walls of the blood vessels.
References
  1. Hickman, C. P., Hickman, F. M., Kats, L. B. (2001). Laboratory Studies in Integrated Principles of Zoology. Tenth Edition. Mc Graw Hill Publishers, New York. 440pp.

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